Teachers:
A. Provenzani, V. Adami, P. Gatto, M. Cardano (University of Trento)
M. Mano (CNC-University of Coimbra)
J. Houser (CEITEC- Masarik University)
The goal of this course is to provide the basis for the understanding of modern biomedical research technologies, including high-throughput screening platforms especially relevant in the target and drug discovery process. In addition, the course will cover knowledge on biophysical techniques used to characterize biomolecular structures/interactions and tools for the development of new experimental models such as cell reprogramming, genome editing and organoids generation.
Upon successful completion of this course, participants should:
- Distinguish among different approaches for Drug Discovery
- Know about paradigm shift in innovation: from closed innovation to open innovation
- Understand the rationale of HTS in academia
- Familiarize with the library formats, miniaturization and Microplates choice
- Understand the principle of liquid handling technologies
- Learn about a pooled screening approach: DNA Encoded Libraries
- Illustrate the requirements of a Bioassay for HTS and the possible classification
- Be able to go through the data analysis process
- List the canonical targets of common drugs and be aware of the druggable space
- Apply correctly biochemical and cell based assays
- Know the basics of target-compound interaction
- List the existing biophysical techniques for molecules interaction
- Familiarize with advanced models for cell-based screening assays: stem cells, iPS cells, 3D models
- Distinguish between target based and phenotypic screening
- Know about High Content Screening: the workflow, instrumentation and software
- Know about the principle of functional genomics
- Familiarize with RNAi screening: arrayed and pooled