E. Biasini, G. Lolli, C. Del Bianco, R. Belli , D. Peroni (University of Trento)
R. Fiala, K. Kubichek, P. Kaderavek, J. Novacek (CEITEC Masaryk University)
The aim of the course is to provide a broad knowledge of methods and applications within Proteomics, exploring advanced methods for protein characterization and functional proteomics. Emphasis will be placed on purification and separation methods, on MS-based strategies and on high-end proteomics workflows and technologies, including NMR, Crystallography and Cryo-EM. Moreover, specific examples of practical applications and case studies will be provided.
Upon successful completion of this course, participants should:
- Understand the principles underlying the expression, purification and characterization of proteins.
- Discuss the importance of using mass spectrometry in the study of proteins in complex biological samples, explaining their potential and limitations.
- Critically choose the proteomic approach for the qualitative and quantitative analysis that is best suited to answer the scientific question of interest.
- Evaluate the appropriate method for sample preparation considering compatibility with mass spectrometry analyses, as well as the purpose of the experiment.
- Understand the principle of operation of the main components of the mass spectrometer, optimizing the parameters that determine its performance.
- Read mass spectra (e.g., de novo sequencing) and understand the logic of complex dataset analyses via software.
- Consult online databases and use the most common software for processing experimental data.
- Interpret, process and present experimental results.
- Understand the principles behind Nuclear Magnetic Resonance (NMR) spectroscopy, X-ray crystallography, and cryoelectron microscopy (CryoEM) for the structural analysis of proteins and protein complexes.
- Read and understand scientific articles in the field of protein analysis.